XTerra Columns – Celebrating 20 Years of Hybrid Technology

XTerra Columns – Celebrating 20 Years of Hybrid Technology


[MUSIC PLAYING] Hi, I’m Bonnie Alden. I’m a principal scientist
at Waters Corporation. I’ve evaluated chromatographic
materials for over 35 years. My original evaluations
were basically on silica-based materials. And that was the
state-of-the-art back in 1997. And we have done a lot of work
in improving silica particles and standard bonding
technologies. So our next landscape to
actually try to improve upon is actually particle technology. And we wanted to
widen the boundaries that chromatographic
people actually were held to at that time. Until XTerra, chromatographers
had to use a certain pH range. They were constrained
by that, especially for silica-based materials. But with XTerra, we actually
did tri-functional bonding, as well as a hybrid
particle, which gave a much stable material. The hybrid material
did allow higher pHs to be used for mobile phases. So that actually opened up
the chromatographic space that a scientist could work in. For myself, the option of
going to a high pH mobile phase gives me another tool in
my method development. I can see what
happens to analytes when you change the pH. And I have a wider
range to use that in. With silica, I’d be probably
operating mostly in maybe pH 3 to maybe pH 5.5, 6. Whereas within XTerra, I could
operate in pHs of maybe 2 to maybe 10 to 12. Moving the pH is
definitely going to change your retention times. Bases might get longer retained. Acids might come out
a little bit earlier. You can actually
change your selectivity within a separation. XTerra was well-received
by the industry. And actually, people
saw the potential in being able to use
higher pH mobile phases. It gave them another tool. We always like to
provide a tool for people to solve other problems. [MUSIC PLAYING]

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